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random hexamer primes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher random hexamer primes
    Random Hexamer Primes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+hexamer+primes/pm39476027-49-29-32
    Average 90 stars, based on 1 article reviews
    random hexamer primes - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: p53 mutants G245S and R337H associated with the Li-Fraumeni syndrome regulate distinct metabolic pathways.
    Article Snippet: Li-Fraumeni and Li-Fraumeni-like syndromes (LFS/LFL) are hereditary cancer predisposition disorders associated with germline mutations in the TP53 tumor suppressor gene.. Here, we stably expressed LFS/ LFL-associated p53 mutants R337H and G245S in p53-null H1299 cells to study their cellular and molecular effects.. Mutant proteins showed distinct oligomerization states and opposing effects on cell proliferation and viability.

    Article Title: Functional characterization of the translation initiation factor eIF4E of Echinococcus granulosus.
    Article Snippet: The eukaryotic initiation factor 4E (eIF4E) specifically recognizes the 5′ mRNA cap, a rate-limiting step in the translation initiation process.. Although the 7-methylguanosine cap (MMGcap) is the most common 5′ cap structure in eukaryotes, the trans-splicing process that occurs in several organism groups, including nematodes and flatworms, leads to the addition of a trimethylguanosine cap (TMGcap) to some RNA transcripts.. In some helminths, eIF4E can have a dual capacity to bind both MMGcap and TMGcap.

    Reverse Transcription:

    Article Title: p53 mutants G245S and R337H associated with the Li-Fraumeni syndrome regulate distinct metabolic pathways.
    Article Snippet: Li-Fraumeni and Li-Fraumeni-like syndromes (LFS/LFL) are hereditary cancer predisposition disorders associated with germline mutations in the TP53 tumor suppressor gene.. Here, we stably expressed LFS/ LFL-associated p53 mutants R337H and G245S in p53-null H1299 cells to study their cellular and molecular effects.. Mutant proteins showed distinct oligomerization states and opposing effects on cell proliferation and viability.

    Article Title: HSV1-induced enhancement of productive HIV-1 replication is associated with interferon pathway downregulation in human macrophages
    Article Snippet: The concentration of total RNA was determined using Qubit Quantitation Fluorometer and Quant-iT reagents (ThermoFisher Scientific, USA). .. The first strand of cDNA was synthesised from 1 μg of total RNA using RT 2 First-Strand Kit (Qiagen, USA) or MMLV Reverse Transcriptase (ThermoFisher Scientific, USA), and 300ng of Random Hexamer primes (ThermoFisher Scientific, USA) as the anchor primer. .. For the qPCR reaction, 1.5 μL of template cDNA (approximately 50 ng) was mixed with 300nM of each primer , and 1x SYBR ® Green qPCR master mix (Applied Biosystems, USA) Alternatively, the mRNA levels for the RFs were evaluated by the RT2 Profiler PCR Array (CAPH12347A) (Qiagen, Germany), composed of primers for different IFN-induced restriction factors, such as APOBEC3G ( Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3G , cat # PPH06904A), IFITM1 ( Interferon Induced Transmembrane Protein 1, cat # PPH05981C), IFITM2 ( Interferon Induced Transmembrane Protein 2 , cat # PPH05548F), IFITM3 ( Interferon Induced Transmembrane Protein 3 , cat # PPH02872E), IFN1-α ( Interferon 1-α , cat# PPH01321B), IFNR ( Interferon Receptor , PPH00869F), MX1 ( Myxovirus Resistance Protein 1 , cat # PPH01325A), MX2 ( Myxovirus Resistance Protein 2, cat # PPH01326F), MCPIP1 ( Monocyte Chemotactic Protein-Induced Protein 1 , cat # PPH16134B), SAMHD1 ( SAM domain and HD domain-containing protein 1 , cat # PPH18140A), TETHERIN / BST-2 (cat # PPH05790B) and the housekeeping gene GAPDH ( Glyceraldehyde 3 - phosphate dehydrogenase, cat# PPH00150F).

    Article Title: Functional characterization of the translation initiation factor eIF4E of Echinococcus granulosus.
    Article Snippet: The eukaryotic initiation factor 4E (eIF4E) specifically recognizes the 5′ mRNA cap, a rate-limiting step in the translation initiation process.. Although the 7-methylguanosine cap (MMGcap) is the most common 5′ cap structure in eukaryotes, the trans-splicing process that occurs in several organism groups, including nematodes and flatworms, leads to the addition of a trimethylguanosine cap (TMGcap) to some RNA transcripts.. In some helminths, eIF4E can have a dual capacity to bind both MMGcap and TMGcap.

    Article Title: HSV1-induced enhancement of productive HIV-1 replication is associated with interferon pathway downregulation in human macrophages.
    Article Snippet: The concentration of total RNA was determined using Qubit Quantitation Fluorometer and QuantiT reagents (ThermoFisher Scientific, USA). .. The first strand of cDNA was synthesised from 1 μg of total RNA using RT2 First-Strand Kit (Qiagen, USA) or MMLV Reverse Transcriptase (ThermoFisher Scientific, USA), and 300ng of Random Hexamer primes (ThermoFisher Scientific, USA) as the anchor primer. (29) For the qPCR reaction, 1.5 μL of template cDNA (approximately 50 ng) was mixed with 300nM of each primer (Table I), and 1x SYBR® Green qPCR master mix (Applied Biosystems, USA) Alternatively, the mRNA levels for the RFs were evaluated by the RT2 Profiler PCR Array (CAPH12347A) (Qiagen, Germany), composed of primers for different IFN-induced restriction factors, such as APOBEC3G (Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3G, cat # PPH06904A), IFITM1 (Interferon Induced Transmembrane Protein 1, cat # PPH05981C), IFITM2 (Interferon Induced Transmembrane Protein 2, cat # PPH05548F), IFITM3 (Interferon Induced Transmembrane Protein 3, cat # PPH02872E), IFN1-α (Interferon 1-α, cat# PPH01321B), IFNR (Interferon Receptor, PPH00869F), MX1 (Myxovirus Resistance Protein 1, cat # PPH01325A), MX2 (Myxovirus Resistance Protein 2, cat # PPH01326F), MCPIP1 (Monocyte Chemotactic Protein-Induced Protein 1, cat # PPH16134B), SAMHD1 (SAM domain and HD domaincontaining protein 1, cat # PPH18140A), TETHERIN / BST-2 (cat # PPH05790B) and the housekeeping gene GAPDH (Glyceraldehyde 3-phosphate dehydrogenase, cat# PPH00150F). .. The qPCR reactions were performed using a 7500 Fast Real-Time PCR system (Applied Biosystems).

    Random Hexamer:

    Article Title: p53 mutants G245S and R337H associated with the Li-Fraumeni syndrome regulate distinct metabolic pathways.
    Article Snippet: Li-Fraumeni and Li-Fraumeni-like syndromes (LFS/LFL) are hereditary cancer predisposition disorders associated with germline mutations in the TP53 tumor suppressor gene.. Here, we stably expressed LFS/ LFL-associated p53 mutants R337H and G245S in p53-null H1299 cells to study their cellular and molecular effects.. Mutant proteins showed distinct oligomerization states and opposing effects on cell proliferation and viability.

    Article Title: HSV1-induced enhancement of productive HIV-1 replication is associated with interferon pathway downregulation in human macrophages
    Article Snippet: The concentration of total RNA was determined using Qubit Quantitation Fluorometer and Quant-iT reagents (ThermoFisher Scientific, USA). .. The first strand of cDNA was synthesised from 1 μg of total RNA using RT 2 First-Strand Kit (Qiagen, USA) or MMLV Reverse Transcriptase (ThermoFisher Scientific, USA), and 300ng of Random Hexamer primes (ThermoFisher Scientific, USA) as the anchor primer. .. For the qPCR reaction, 1.5 μL of template cDNA (approximately 50 ng) was mixed with 300nM of each primer , and 1x SYBR ® Green qPCR master mix (Applied Biosystems, USA) Alternatively, the mRNA levels for the RFs were evaluated by the RT2 Profiler PCR Array (CAPH12347A) (Qiagen, Germany), composed of primers for different IFN-induced restriction factors, such as APOBEC3G ( Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3G , cat # PPH06904A), IFITM1 ( Interferon Induced Transmembrane Protein 1, cat # PPH05981C), IFITM2 ( Interferon Induced Transmembrane Protein 2 , cat # PPH05548F), IFITM3 ( Interferon Induced Transmembrane Protein 3 , cat # PPH02872E), IFN1-α ( Interferon 1-α , cat# PPH01321B), IFNR ( Interferon Receptor , PPH00869F), MX1 ( Myxovirus Resistance Protein 1 , cat # PPH01325A), MX2 ( Myxovirus Resistance Protein 2, cat # PPH01326F), MCPIP1 ( Monocyte Chemotactic Protein-Induced Protein 1 , cat # PPH16134B), SAMHD1 ( SAM domain and HD domain-containing protein 1 , cat # PPH18140A), TETHERIN / BST-2 (cat # PPH05790B) and the housekeeping gene GAPDH ( Glyceraldehyde 3 - phosphate dehydrogenase, cat# PPH00150F).

    Article Title: Functional characterization of the translation initiation factor eIF4E of Echinococcus granulosus.
    Article Snippet: The eukaryotic initiation factor 4E (eIF4E) specifically recognizes the 5′ mRNA cap, a rate-limiting step in the translation initiation process.. Although the 7-methylguanosine cap (MMGcap) is the most common 5′ cap structure in eukaryotes, the trans-splicing process that occurs in several organism groups, including nematodes and flatworms, leads to the addition of a trimethylguanosine cap (TMGcap) to some RNA transcripts.. In some helminths, eIF4E can have a dual capacity to bind both MMGcap and TMGcap.

    Article Title: HSV1-induced enhancement of productive HIV-1 replication is associated with interferon pathway downregulation in human macrophages.
    Article Snippet: The concentration of total RNA was determined using Qubit Quantitation Fluorometer and QuantiT reagents (ThermoFisher Scientific, USA). .. The first strand of cDNA was synthesised from 1 μg of total RNA using RT2 First-Strand Kit (Qiagen, USA) or MMLV Reverse Transcriptase (ThermoFisher Scientific, USA), and 300ng of Random Hexamer primes (ThermoFisher Scientific, USA) as the anchor primer. (29) For the qPCR reaction, 1.5 μL of template cDNA (approximately 50 ng) was mixed with 300nM of each primer (Table I), and 1x SYBR® Green qPCR master mix (Applied Biosystems, USA) Alternatively, the mRNA levels for the RFs were evaluated by the RT2 Profiler PCR Array (CAPH12347A) (Qiagen, Germany), composed of primers for different IFN-induced restriction factors, such as APOBEC3G (Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3G, cat # PPH06904A), IFITM1 (Interferon Induced Transmembrane Protein 1, cat # PPH05981C), IFITM2 (Interferon Induced Transmembrane Protein 2, cat # PPH05548F), IFITM3 (Interferon Induced Transmembrane Protein 3, cat # PPH02872E), IFN1-α (Interferon 1-α, cat# PPH01321B), IFNR (Interferon Receptor, PPH00869F), MX1 (Myxovirus Resistance Protein 1, cat # PPH01325A), MX2 (Myxovirus Resistance Protein 2, cat # PPH01326F), MCPIP1 (Monocyte Chemotactic Protein-Induced Protein 1, cat # PPH16134B), SAMHD1 (SAM domain and HD domaincontaining protein 1, cat # PPH18140A), TETHERIN / BST-2 (cat # PPH05790B) and the housekeeping gene GAPDH (Glyceraldehyde 3-phosphate dehydrogenase, cat# PPH00150F). .. The qPCR reactions were performed using a 7500 Fast Real-Time PCR system (Applied Biosystems).

    Recombinant:

    Article Title: p53 mutants G245S and R337H associated with the Li-Fraumeni syndrome regulate distinct metabolic pathways.
    Article Snippet: Li-Fraumeni and Li-Fraumeni-like syndromes (LFS/LFL) are hereditary cancer predisposition disorders associated with germline mutations in the TP53 tumor suppressor gene.. Here, we stably expressed LFS/ LFL-associated p53 mutants R337H and G245S in p53-null H1299 cells to study their cellular and molecular effects.. Mutant proteins showed distinct oligomerization states and opposing effects on cell proliferation and viability.

    Real-time Polymerase Chain Reaction:

    Article Title: p53 mutants G245S and R337H associated with the Li-Fraumeni syndrome regulate distinct metabolic pathways.
    Article Snippet: Li-Fraumeni and Li-Fraumeni-like syndromes (LFS/LFL) are hereditary cancer predisposition disorders associated with germline mutations in the TP53 tumor suppressor gene.. Here, we stably expressed LFS/ LFL-associated p53 mutants R337H and G245S in p53-null H1299 cells to study their cellular and molecular effects.. Mutant proteins showed distinct oligomerization states and opposing effects on cell proliferation and viability.

    Article Title: HSV1-induced enhancement of productive HIV-1 replication is associated with interferon pathway downregulation in human macrophages.
    Article Snippet: The concentration of total RNA was determined using Qubit Quantitation Fluorometer and QuantiT reagents (ThermoFisher Scientific, USA). .. The first strand of cDNA was synthesised from 1 μg of total RNA using RT2 First-Strand Kit (Qiagen, USA) or MMLV Reverse Transcriptase (ThermoFisher Scientific, USA), and 300ng of Random Hexamer primes (ThermoFisher Scientific, USA) as the anchor primer. (29) For the qPCR reaction, 1.5 μL of template cDNA (approximately 50 ng) was mixed with 300nM of each primer (Table I), and 1x SYBR® Green qPCR master mix (Applied Biosystems, USA) Alternatively, the mRNA levels for the RFs were evaluated by the RT2 Profiler PCR Array (CAPH12347A) (Qiagen, Germany), composed of primers for different IFN-induced restriction factors, such as APOBEC3G (Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3G, cat # PPH06904A), IFITM1 (Interferon Induced Transmembrane Protein 1, cat # PPH05981C), IFITM2 (Interferon Induced Transmembrane Protein 2, cat # PPH05548F), IFITM3 (Interferon Induced Transmembrane Protein 3, cat # PPH02872E), IFN1-α (Interferon 1-α, cat# PPH01321B), IFNR (Interferon Receptor, PPH00869F), MX1 (Myxovirus Resistance Protein 1, cat # PPH01325A), MX2 (Myxovirus Resistance Protein 2, cat # PPH01326F), MCPIP1 (Monocyte Chemotactic Protein-Induced Protein 1, cat # PPH16134B), SAMHD1 (SAM domain and HD domaincontaining protein 1, cat # PPH18140A), TETHERIN / BST-2 (cat # PPH05790B) and the housekeeping gene GAPDH (Glyceraldehyde 3-phosphate dehydrogenase, cat# PPH00150F). .. The qPCR reactions were performed using a 7500 Fast Real-Time PCR system (Applied Biosystems).

    SYBR Green Assay:

    Article Title: HSV1-induced enhancement of productive HIV-1 replication is associated with interferon pathway downregulation in human macrophages.
    Article Snippet: The concentration of total RNA was determined using Qubit Quantitation Fluorometer and QuantiT reagents (ThermoFisher Scientific, USA). .. The first strand of cDNA was synthesised from 1 μg of total RNA using RT2 First-Strand Kit (Qiagen, USA) or MMLV Reverse Transcriptase (ThermoFisher Scientific, USA), and 300ng of Random Hexamer primes (ThermoFisher Scientific, USA) as the anchor primer. (29) For the qPCR reaction, 1.5 μL of template cDNA (approximately 50 ng) was mixed with 300nM of each primer (Table I), and 1x SYBR® Green qPCR master mix (Applied Biosystems, USA) Alternatively, the mRNA levels for the RFs were evaluated by the RT2 Profiler PCR Array (CAPH12347A) (Qiagen, Germany), composed of primers for different IFN-induced restriction factors, such as APOBEC3G (Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3G, cat # PPH06904A), IFITM1 (Interferon Induced Transmembrane Protein 1, cat # PPH05981C), IFITM2 (Interferon Induced Transmembrane Protein 2, cat # PPH05548F), IFITM3 (Interferon Induced Transmembrane Protein 3, cat # PPH02872E), IFN1-α (Interferon 1-α, cat# PPH01321B), IFNR (Interferon Receptor, PPH00869F), MX1 (Myxovirus Resistance Protein 1, cat # PPH01325A), MX2 (Myxovirus Resistance Protein 2, cat # PPH01326F), MCPIP1 (Monocyte Chemotactic Protein-Induced Protein 1, cat # PPH16134B), SAMHD1 (SAM domain and HD domaincontaining protein 1, cat # PPH18140A), TETHERIN / BST-2 (cat # PPH05790B) and the housekeeping gene GAPDH (Glyceraldehyde 3-phosphate dehydrogenase, cat# PPH00150F). .. The qPCR reactions were performed using a 7500 Fast Real-Time PCR system (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: HSV1-induced enhancement of productive HIV-1 replication is associated with interferon pathway downregulation in human macrophages.
    Article Snippet: The concentration of total RNA was determined using Qubit Quantitation Fluorometer and QuantiT reagents (ThermoFisher Scientific, USA). .. The first strand of cDNA was synthesised from 1 μg of total RNA using RT2 First-Strand Kit (Qiagen, USA) or MMLV Reverse Transcriptase (ThermoFisher Scientific, USA), and 300ng of Random Hexamer primes (ThermoFisher Scientific, USA) as the anchor primer. (29) For the qPCR reaction, 1.5 μL of template cDNA (approximately 50 ng) was mixed with 300nM of each primer (Table I), and 1x SYBR® Green qPCR master mix (Applied Biosystems, USA) Alternatively, the mRNA levels for the RFs were evaluated by the RT2 Profiler PCR Array (CAPH12347A) (Qiagen, Germany), composed of primers for different IFN-induced restriction factors, such as APOBEC3G (Apolipoprotein B MRNA Editing Enzyme Catalytic Subunit 3G, cat # PPH06904A), IFITM1 (Interferon Induced Transmembrane Protein 1, cat # PPH05981C), IFITM2 (Interferon Induced Transmembrane Protein 2, cat # PPH05548F), IFITM3 (Interferon Induced Transmembrane Protein 3, cat # PPH02872E), IFN1-α (Interferon 1-α, cat# PPH01321B), IFNR (Interferon Receptor, PPH00869F), MX1 (Myxovirus Resistance Protein 1, cat # PPH01325A), MX2 (Myxovirus Resistance Protein 2, cat # PPH01326F), MCPIP1 (Monocyte Chemotactic Protein-Induced Protein 1, cat # PPH16134B), SAMHD1 (SAM domain and HD domaincontaining protein 1, cat # PPH18140A), TETHERIN / BST-2 (cat # PPH05790B) and the housekeeping gene GAPDH (Glyceraldehyde 3-phosphate dehydrogenase, cat# PPH00150F). .. The qPCR reactions were performed using a 7500 Fast Real-Time PCR system (Applied Biosystems).



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